Nano-ESI-MS/MS
Identification on Apoptosis Associated Proteins Induced by Inhibiting
Ubiquitin-proteasome Pathway
WANG Hong-Xia, JIN Bao-Feng1,
WANG Jie, HE Kun, YANG Song-Cheng, SHEN Bei-Feng1, ZHANG Xue-Min*
( National Center of Biomedical Analysis, Academy of Military Medical
Sciences, Beijing 100850, China;
1 Institute of Basic Medical Science, Academy of Military Medical
Sciences, Beijing 100850, China )
Abstract
CapLC-ESI-MS/MS and nano-ESI-MS/MS techniques were used to identify the
apoptosis associated proteins induced by inhibiting the ubiquitin-proteasome
pathway in Mo7e leukaemic cells. In 2-DE, spot H was found to initiate its
overexpression at 2 h after the inhibition and reached its peak at 6 h. It was
identified as Rho GDI &bgr; protein after the tandem mass spectrum and
after the sequence of its tryptic peptides were obtained by the ESI-MS/MS techniques.
It was not revealed by peptide mass fingerprint using MALDI-TOF-MS. Other two
spots induced by the inhibition appeared close to spot H were also revealed
identical to Rho GDI &brg;, possibly due to unknown modifications.
Key words proteomics; nanoESIMS/MS; apoptosis;
ubiquitinproteasome pathway
*Corresponding author: Tel,
86-10-66930169; Fax, 86-10-62751842; e-mail, [email protected]